Classification of laboratories according to use
It is generally accepted that three or four kinds of microbiology laboratories are required. In the WHO classification these are; Biosafety Level 1 to 4 for Risk Groups 1 to 4 microorganisms respectively.
Biosafety level
1 2 3 4
Isolation of laboratory No No Op Yes
Room sealable for decontamination No No Yes Yes
Ventilation;
inward air flow No Op Yes Yes
mechanical via building system No Op Op No
mechanical, independent No No Op Yes
filtered air exhaust No No Op Yes
Double door entry No No Yes Yes
Airlock No No No Yes
Airlock with shower No No No Yes
Effluent treatment No No No Yes
Autoclave on site Yes Yes Yes Yes
in laboratory room No No Yes Yes
double ended No No Op Yes
Biological safety cabinets
Classes I or II No Yes Yes Op
Class III No No Op Yes
A problem which should be mentioned is that although these classifications may be reasonable for clinical and research laboratories, they are hardly applicable to many industrial, food science and agricultural laboratories.
Animal House Containment and biosafety levels
The rules for animal houses parallel those for laboratories. Consideration should be given to the requirements of individual species such as their behavior, zoonoses and parasites.
Level | 1 | As for Level 1 laboratories plus limited access, arthropod control, protective clothing and gloves |
Level | 2 | As for Level 1 laboratories and animal houses plus decontamination of waste and cages before washing, microbiological safety cabinets and personal protection devices |
Level | 3 | As for Level 2 laboratories and animal houses plus controlled access |
Level | 4 | As for Level 3 laboratories and animal houses plus strictly limited access, waste decontaminated before removal, and clothes changing room and shower |